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- 抗原 See all AGT 抗体
- AGT (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))
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抗原表位
- Internal Region
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适用
- 人, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This AGT antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human AGT / Angiotensinogen. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%) Marmoset (85%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 13 amino acid peptide from internal region of human AGT / Angiotensinogen. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Marmoset (85%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product AGT Primary Antibody
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anti-Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) antibody
AGT 适用: 人 WB, ICC, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) (AA 23-477) antibodyAGT 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) (AA 25-477) antibodyAGT 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) (AA 376-485) antibodyAGT 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A10 unconjugated
anti-Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) (AA 25-477) antibodyAGT 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) (AA 44-427) antibodyAGT 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) antibodyAGT 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) (AA 35-285) antibodyAGT 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) antibodyAGT 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) antibodyAGT 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (2.5 - 5 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- AGT (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))
- 别名
- AGT / Angiotensinogen (AGT 产品)
- 别名
- ANHU antibody, SERPINA8 antibody, AI265500 antibody, AngI antibody, AngII antibody, Aogen antibody, Serpina8 antibody, ANRT antibody, Ang antibody, PAT antibody, wu:fb62f06 antibody, wu:fj87b02 antibody, zgc:111892 antibody, AGT antibody, angt antibody, ANGT antibody, angiotensinogen antibody, angiotensinogen (serpin peptidase inhibitor, clade A, member 8) antibody, AGT antibody, Agt antibody, agt antibody
- 背景
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Name/Gene ID: AGT
Family: Hormone
Synonyms: AGT, Angiotensin I, ANHU, Angiotensinogen, Pre-angiotensinogen, Angiotensin II, SERPINA8, Serpin A8 - 基因ID
- 183
- 途径
- JAK/STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Monocarboxylic Acid Catabolic Process, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Regulation of long-term Neuronal Synaptic Plasticity
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