(1 reference)
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- 抗原 See all IL-6 (IL6) 抗体
- IL-6 (IL6) (Interleukin 6 (IL6))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IL-6 antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 209-403-310
- Supplier
- Rockland
- 原理
- IL-6 Antibody Peroxidase Conjugated
- 交叉反应 （详细）
- In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human Il-6 in cell supernatants and certain body fluids.
- 产品特性
- Synonyms: rabbit Anti-IL-6 peroxidase conjugated antibody, rabbit anti-interleukin-6 peroxidase conjugated antibody, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF
- 纯化方法
- This purified antibody has been heated to 56°C for 30 minutes.
- 免疫原
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Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-6 produced in E.coli.
Immunogen Type: Recombinant Protein
- 亚型
- IgG
- Top Product
- Discover our top product IL6 Primary Antibody
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anti-Interleukin 6 (IL6) (AA 111-150) antibody
IL6 适用: 人, 小鼠, 大鼠, 兔, Cow WB, ELISA, FACS, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 6 (IL6) antibodyIL6 适用: 人 ELISA, FACS, Func, ELISpot 宿主: 小鼠 Monoclonal B-E8 unconjugated
anti-Interleukin 6 (IL6) (AA 21-211) antibodyIL6 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Interleukin 6 (IL6) (AA 27-212) antibodyIL6 适用: 猕猴 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 18# unconjugated
anti-Interleukin 6 (IL6) (AA 21-211) antibodyIL6 适用: 大鼠 WB, IHC, IP, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Interleukin 6 (IL6) (AA 101-150) antibodyIL6 适用: 小鼠, 大鼠 WB, ELISA, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 6 (IL6) (AA 27-241) antibodyIL6 适用: 兔 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C305 unconjugated
anti-Interleukin 6 (IL6) (AA 25-211) antibodyIL6 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 6 (IL6) (AA 27-55) antibodyIL6 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 6 (IL6) (AA 26-50) antibodyIL6 适用: 人, 大鼠 ELISA, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:500-1:2,500
Application Note: This purified antibody has been tested in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/μl by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:1,000-1:5,000
ELISA Dilution: 1:10,000-1:50,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.1 mg/ml
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Current concepts in the management of renovascular hypertension and ischemic renal failure." in: American journal of kidney diseases : the official journal of the National Kidney Foundation, Vol. 13, Issue 6 Suppl 1, pp. 33-7, (1989) (PubMed).
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: "Current concepts in the management of renovascular hypertension and ischemic renal failure." in: American journal of kidney diseases : the official journal of the National Kidney Foundation, Vol. 13, Issue 6 Suppl 1, pp. 33-7, (1989) (PubMed).
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- 抗原
- IL-6 (IL6) (Interleukin 6 (IL6))
- 别名
- IL6 (IL6 产品)
- 背景
- Background: IL-6 is a secreted cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
- 基因ID
- 3569
- NCBI登录号
- NP_000591
- UniProt
- P05231
- 途径
- TLR signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
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