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LIM Domain Binding 1 Protein 抗体 (AA 361-373)

LDB1 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN103458
发货至: 中国
  • 抗原 See all LIM Domain Binding 1 Protein (LDB1) 抗体
    LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))
    抗原表位
    • 15
    • 11
    • 8
    • 7
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 361-373
    适用
    • 53
    • 9
    • 7
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
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    小鼠
    宿主
    • 51
    • 4
    克隆类型
    • 52
    • 3
    多克隆
    标记
    • 27
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This LIM Domain Binding 1 Protein antibody is un-conjugated
    应用范围
    • 51
    • 25
    • 13
    • 13
    • 9
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    过滤
    Sterile filtered
    免疫原
    This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 361-373 of mouse LDB1 protein.
    亚型
    IgG
    Top Product
    Discover our top product LDB1 Primary Antibody
  • 应用备注
    This affinity purified antibody has been tested for use in ELISA, western blot and CHIP.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB1 by western blotting in the appropriate cell lysate or extract. This antibody has been used in a ChIP assay using murine erythroleukemia (MEL) cells. The test sequence was the upstream enhancer of the GATA-1 gene a putative LDB1 binding region as suggested by Orkin et al.   Anti-LDB1 was used successfully in ChIP assays to precipitate a roughly 4-fold enrichment at the GATA1-HS1 enhancer element in DMSO-induced murine erythroleukemia cells.  We suggest using 20µg for 10E8 cells for ChIP.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.80 mg/mL
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
  • 抗原
    LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))
    别名
    LDB1 (LDB1 产品)
    别名
    CLIM2 antibody, NLI antibody, chi antibody, clim2 antibody, nli antibody, xldb1 antibody, ldb1 antibody, LDB1 antibody, ldb4 antibody, ldb4b antibody, LIM domain binding 1 antibody, LIM domain binding 1 L homeolog antibody, LIM-domain binding 1b antibody, LIM domain binding protein 1 antibody, LIM domain binding 1a antibody, Ldb1 antibody, LDB1 antibody, ldb1.L antibody, ldb1b antibody, xldb1 antibody, ldb1a antibody
    背景
    LDB1 is also known as CLIM 2, LIM Domain Binding 1, NLI and Nuclear LIM Domain Interactor. The LIM-domain binding protein binds to the LIM domain of LIM homeodomain proteins which are transcriptional regulators of development. Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB1) is located in the nuclei of neuronal cells during development, it is co-expressed with Isl1 in early motor neuron differentiation and has a suggested role in the Isl1 dependent development of motor neurons. It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors, both of which have essential roles in development.
    Synonyms: LDB1, CLIM 2, LIM Domain Binding 1, NLI and Nuclear LIM Domain Interactor.
    基因ID
    16825, 6754520
    UniProt
    P70662
    途径
    Stem Cell Maintenance, Chromatin Binding
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