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MTOR ELISA 试剂盒 (Mechanistic Target of Rapamycin (serine/threonine Kinase))

Details for Product FRAP1 ELISA Kit No. ABIN2748394, 供应商: Log in to see
抗原
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Alternatives
人 MTOR ELISA Kit
抗原表位
pSer2448
适用

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15
12
1
1
1
1
1
1
1
1
实验类型
Cell ELISA
应用范围
ELISA
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原理 Cell-Based Human mTOR (S2448) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.
样品类型 Cell Culture Cells
Analytical Method Semi-Quantitative
检测方法 Colorimetric
特异性 The antibodies provided in this kit recognizes human and mouse mTOR phosphorylated at Serine-2448 and total mTOR for comparison.
产品特性
  • Site and signal pathway-specific
  • In vitro detection of adherent cell culture
  • No sample lysis needed
  • Compatible with a standard ELISA plate reader
  • Faster results than with ELISA
  • Adaptable for high-throughput screening and drug discovery
组件
  • uncoated 96-well Microplate
  • Wash Buffer A
  • Wash Buffer B
  • Fixing Solution
  • Quenching Buffer
  • Blocking Buffer
  • Anti-phospho antibody
  • Anti-pan antibody
  • HRP-Conjugated Secondary Antibody
  • TMB One-Step Substrate
  • Stop Solution
  • 试剂未包括
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    别名 mTOR (FRAP1 ELISA Kit 摘要)
    背景 Mechanistic target of rapamycin, mammalian target of rapamycin (mTOR), FK506-binding protein 12-rapamycin-associated protein 1 (FRAP1)
    基因ID 2475
    UniProt P42345
    研究领域 Cardiovascular, Cardiogenesis, Cell Cycle, DNA/RNA
    途径 PI3K-Akt Signaling, RTK signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events
    样本量 100 μL
    板类型 Uncoated
    实验流程
    1. Seed 10,000-30,000 cells into each well and incubate overnight.
    2. Apply various treatment, inhibitors or activators according to manufacture's instructions.
    3. Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.
    4. Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.
    5. Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.
    6. Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
    7. Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    实验流程

    Seed 10,000-30,000 cells into each well and incubate overnight.
    Apply various treatment, inhibitors or activators according to manufacture's instructions.
    Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.
    Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.
    Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.
    Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
    Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    限制 仅限研究用
    储存条件 -20 °C
    储存方法 The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
    有效期 6 months