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PCNA 抗体 (AA 151-261)

Name: PCNA 抗体 (AA 151-261)
SKU: ABIN676583
Availability: OutOfStock

PCNA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Mouse spleen lysates probed with PCNA Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

Formalin-fixed and paraffin embedded human brain tissue labeled with Anti-PCNA Polyclonal Antibody, Unconjugated (ABIN676583) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti-PCNA Polyclonal Antibody, Unconjugated (ABIN676583) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Formalin-fixed and paraffin embedded human oral squamous cell carcinoma labeled with Anti- PCNA-Proliferation Marker Polyclonal Antibody, Unconjugated (ABIN676583) at 1:100 followed by conjugation to the secondary antibody and DAB staining

IHC

IHC (p)

Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti-PCNA Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC (p)

6 images

(6 references)

产品编号 ABIN676583

发货至: 中国

Datasheet as PDF

抗原 See all PCNA 抗体

PCNA (Proliferating Cell Nuclear Antigen (PCNA))
抗原表位
16

16

16

14

13

10

8

6

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 151-261
适用
216

152

151

57

32

28

23

19

19

18

18

14

11

11

7

5

5

4

4

4

4

3

3

3

2

2

2

2

2

1

1

1

1

1
人, 小鼠, 大鼠
宿主
142

87

3
兔
Compatible Secondaries
克隆类型
141

90

1
多克隆
标记
116

17

14

9

5

5

5

5

5

5

5

5

5

5

5

5

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This PCNA antibody is un-conjugated
应用范围
202

67

65

59

59

49

48

39

31

16

14

11

5

4

3

2

2

2

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, 大鼠

预测反应

Cow,Sheep,Pig,Chicken,Rabbit,Guinea Pig

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human PCNA

亚型

IgG
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibody
PCNA 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 1G7 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibody
PCNA 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal S1 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibody
PCNA 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) antibody
PCNA 适用: 人 WB, IHC, IF, IHC (p), IP, ICC, IHC (fro), FISH 宿主: 兔 Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 53-196) antibody
PCNA 适用: 人, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 7H4F8 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 201-261) antibody
PCNA 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 6-261) antibody
PCNA 适用: 人, 小鼠, 大鼠, Pig, 犬, 兔, 猕猴 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) antibody
PCNA 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1H10 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) antibody
PCNA 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 231-261), (C-Term) antibody
PCNA 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB17486 unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Zhao, Han, Hong, Sun: "Adipose differentiation‑related protein knockdown inhibits vascular smooth muscle cell proliferation and migration and attenuates neointima formation." in: Molecular medicine reports, Vol. 16, Issue 3, pp. 3079-3086, (2018) (PubMed).

Zhou, Zhang, Gao, Tan, Zheng, Zhao, Zhang: "Induced pluripotent stem cell-conditioned medium suppresses pulmonary fibroblast-to-myofibroblast differentiation via the inhibition of TGF-β1/Smad pathway." in: International journal of molecular medicine, Vol. 41, Issue 1, pp. 473-484, (2018) (PubMed).

Zhao, Feng, Zhang, Kou, Li, Liu, Zhang, Cui, Chu, Shen, Min: "Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production." in: Theriogenology, Vol. 89, pp. 280-288, (2017) (PubMed).

Ji, Liu, Zhao, Guo, Xia, Lu, Yin, Xu: "Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3‑E1 cells." in: Molecular medicine reports, Vol. 16, Issue 5, pp. 7391-7397, (2017) (PubMed).

Liang, Peng, Li, Yang, Xie, Li, Du, Zhang: "Silencing of CXCR4 sensitizes triple-negative breast cancer cells to cisplatin." in: Oncotarget, Vol. 6, Issue 2, pp. 1020-30, (2015) (PubMed).
抗原

PCNA (Proliferating Cell Nuclear Antigen (PCNA))

别名

PCNA (PCNA 产品)

背景

Synonyms: ATLD2, Proliferating cell nuclear antigen, PCNA, Cyclin
Background: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

基因ID

5111

UniProt

P12004

途径

Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy