(3 references)-
- 抗原 See all MDM2 抗体
- MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
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抗原表位
- AA 343-358, C-Term
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MDM2 antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 原理
- Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase Mdm2(MDM2) detection. Tested with WB in Human,Mouse,Rat.
- 序列
- EKAKLENSTQ AEEGFD
- 交叉反应 （详细）
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase Mdm2(MDM2) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: MDM2 oncogene, E3 ubiquitin protein ligase
Protein Name: E3 ubiquitin-protein ligase Mdm2 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human MDM2(343-358aa EKAKLENSTQAEEGFD), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product MDM2 Primary Antibody
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anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 101-200) antibody
MDM2 适用: 人 WB, ELISA, IHC (p), RNAi, PLA 宿主: 小鼠 Monoclonal 1A7 unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibodyMDM2 适用: 人, 小鼠 WB, IF, IHC (p), IP, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (pSer166) antibodyMDM2 适用: 人, 小鼠, 猴 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 26-169) antibodyMDM2 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 3C1E3 unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 393-424) antibodyMDM2 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (pSer186) antibodyMDM2 适用: 人 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) antibodyMDM2 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) antibodyMDM2 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 119-438) antibodyMDM2 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1E6 unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 200-430) antibodyMDM2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Sodium azide, Thimerosal (Merthiolate)
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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: "Fra-1 is downregulated in cervical cancer tissues and promotes cervical cancer cell apoptosis by p53 signaling pathway in vitro." in: International journal of oncology, Vol. 46, Issue 4, pp. 1677-84, (2015) (PubMed).
: "The oncoprotein HBXIP modulates the feedback loop of MDM2/p53 to enhance the growth of breast cancer." in: The Journal of biological chemistry, Vol. 290, Issue 37, pp. 22649-61, (2015) (PubMed).
: "CD38 is highly expressed and affects the PI3K/Akt signaling pathway in cervical cancer." in: Oncology reports, Vol. 32, Issue 6, pp. 2703-9, (2014) (PubMed).
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: "Fra-1 is downregulated in cervical cancer tissues and promotes cervical cancer cell apoptosis by p53 signaling pathway in vitro." in: International journal of oncology, Vol. 46, Issue 4, pp. 1677-84, (2015) (PubMed).
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- 抗原
- MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
- 别名
- MDM2 (MDM2 产品)
- 别名
- ACTFS antibody, HDMX antibody, hdm2 antibody, zgc:109834 antibody, 1700007J15Rik antibody, AA415488 antibody, Mdm-2 antibody, MDM2 proto-oncogene antibody, MDM2 proto-oncogene S homeolog antibody, Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) antibody, MDM2 oncogene, E3 ubiquitin protein ligase antibody, transformed mouse 3T3 cell double minute 2 antibody, MDM2 antibody, mdm2.S antibody, Mdm2 antibody, mdm2 antibody
- 背景
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Mdm2 is an important negative regulator of the p53 tumor suppressor. It is the name of a gene as well as the protein encoded by that gene. Mdm2 protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain(TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al.(1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.
Synonyms: Double minute 2 protein antibody|E3 ubiquitin-protein ligase Mdm2 antibody|Hdm 2 antibody|HDM2 antibody|MDM 2 antibody|MDM2 antibody|Mdm2 transformed 3T3 cell double minute 2 p53 binding protein(mouse) binding protein 104 kDa antibody|MDM2_HUMAN antibody|MDM2BP antibody|Mouse Double Minute 2 antibody|MTBP antibody|Murine Double Minute Chromosome 2 antibody|Oncoprotein Mdm2 antibody|p53 Binding Protein Mdm2 antibody|p53-binding protein Mdm2 antibody|Ubiquitin protein ligase E3 Mdm2 antibody - UniProt
- Q00987
- 途径
- p53 Pathway, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway
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