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DYKDDDDK 标记 抗体

ABIN387849 产品详细信息, 供应商: Log in to see
抗原
适用
标记
240
101
39
18
18
9
8
7
4
3
2
1
1
1
宿主

174
109
34
34
17
克隆类型 (克隆位点)
多克隆 ()
标记
非结合性
38
21
16
8
8
8
8
6
6
5
5
5
5
5
5
5
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
应用范围
Western Blotting (WB)
289
131
127
103
85
58
43
14
11
8
7
7
4
4
4
4
3
3
3
2
1
1
1
1
1
1
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免疫原 KLH conjugated synthetic peptide (CDYKDDDDKDYKDDDDK) was used as antigen.
序列 DYKDDDDK
克隆位点 RB0493
亚型 Ig
特异性 KLH conjugated synthetic peptide (CDYKDDDDKDYKDDDDK) was used as antigen.
纯化方法 This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
物质类 Tag
背景 Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion (1).
Synonyms: Tag from influenza hemagglutinin protein
分子量 DA
应用备注 WB = 1:1000
限制 仅限研究用
状态 Liquid
浓度 2 mg/mL
缓冲液 PBS with 0.09 % (W/V) sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
有效期 6 months
厂商提供的图像
Western Blotting (WB) image for anti-DYKDDDDK 标记 抗体 (ABIN387849) Western blot analysis of anti-tag-DYKDDDDK.2x Pab (ABIN387849) in 12tag cell line lys...
背景 Chubet, Brizzard: "Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells." in: BioTechniques, Vol. 20, Issue 1, pp. 136-41, 1996 (PubMed).