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GFP 抗体 (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN285794, 供应商: Log in to see
抗原
  • green fluorescent protein
  • gfp
适用
Aequorea victoria
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宿主

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克隆类型
多克隆
标记
This GFP antibody is un-conjugated
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应用范围
Immunoprecipitation (IP), Western Blotting (WB)
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Available images

免疫原 GFP antibody was raised in rabbit using E. Coli-expressed full length green fluorescent protein as the immunogen.
特异性 This antibody neutralizes and binds to human interferon alpha receptor with high affinity. It interacts with extracellular domain and blocks biological action of Type I interferons.
交叉反应 (详细) This antibody reacts with wild -type GFP and it's variants, such as EGFP, EBFP
纯化方法 purified
别名 GFP
物质类 Tag
背景 The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression.
研究领域 Tags/Labels
应用备注 IP: 1:200-1:500, WB: 1:5,000
Optimal conditions should be determined by the investigator.
限制 仅限研究用
状态 Lyophilized
溶解方式 Reconstitue in distilled water.
浓度 Lot specific
缓冲液 Supplied as a lyophilized Potein A purified rabbit IgG with 0.1 % NaN3
储存液 Sodium azide
注意事项 This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
注意事项 Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.
储存条件 RT/-20 °C
储存方法 Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.
厂商提供的图像
Western Blotting (WB) image for anti-GFP 抗体 (Green Fluorescent Protein) (ABIN285794) Lane 1 - Marker; Lane - GP fusion protein; Lane 3 - Control
有引用在: Orozco, Tahirovic, Rentzsch, Schwenk, Haass, Edbauer: "Loss of fused in sarcoma (FUS) promotes pathological Tau splicing." in: EMBO reports, Vol. 13, Issue 8, pp. 759-64, 2012 (PubMed).