CTCF 抗体 (CCCTC-Binding Factor (Zinc Finger Protein)) (N-Term)

Details for Product anti-CTCF Antibody No. ABIN2780790, 供应商: Log in to see
抗原
  • im:7152666
  • CTCF
  • xctcf
  • AW108038
  • CCCTC-binding factor (zinc finger protein)
  • CCCTC-binding factor
  • CTCF
  • ctcf
  • LOC100224119
  • Ctcf
抗原表位
N-Term
14
7
6
5
2
2
2
1
1
1
1
1
1
1
1
适用
牛, 犬, 豚鼠, 马, 人, 小鼠, 兔, 大鼠
89
45
30
12
10
8
6
6
4
3
3
2
2
1
1
1
宿主

60
27
6
1
1
克隆类型
多克隆
应用范围
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
79
29
27
16
14
11
9
6
3
2
1
1
选项
Supplier
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Supplier Product No.
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免疫原 The immunogen is a synthetic peptide directed towards the N terminal region of human CTCF
Blocking Peptide Blocking peptide for this product available: ABIN975410
序列 MEGDAVEAIV EESETFIKGK ERKTYQRRRE GGQEEDACHL PQNQTDGGEV
预测反应 Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%
产品特性 This is a rabbit polyclonal antibody against CTCF. It was validated on Western Blot using a cell lysate as a positive control.
纯化方法 Affinity Purified
别名 CTCF (CTCF Antibody 摘要)
背景 CTCF is a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in CTCF have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.
Protein Interaction Partner: ZMYM4, UBC, EED, BMI1, KANSL1, CDK19, ZMYM2, CAND1, ZBTB33, TFAP4, SUMO1, SUMO2, SUMO3, SIRT7, TAF3, SMC3, POLR2A, SUZ12, Ctcf, HIST2H2AC, TOP2A, TAF1, NPM1, LMNA, KPNA4, KPNA2, H2AFZ, PARP1, TRB, RXRA, SMAD3, SIN3A, SMC1A, RAD21, YY1, THRB, SMAD4, HMGB1,
Protein Size: 727
分子量 83 kDa
基因ID 10664
NCBI登录号 NM_006565, NP_006556
UniProt P49711
应用备注 Optimal working dilutions should be determined experimentally by the investigator.
说明

Antigen size: 727 AA

限制 仅限研究用
状态 Liquid
浓度 Lot specific
缓冲液 Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
厂商提供的图像
Chromatin Immunoprecipitation (ChIP) image for anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (N-Term) antibody (ABIN2780790) Chromatin Immunoprecipitation (ChIP) Using CTCF Antibody - N-terminal region (ARP3882...
背景 Defossez, Kelly, Filion, Pérez-Torrado, Magdinier, Menoni, Nordgaard, Daniel, Gilson: "The human enhancer blocker CTC-binding factor interacts with the transcription factor Kaiso." in: The Journal of biological chemistry, Vol. 280, Issue 52, pp. 43017-23, 2005 (PubMed).

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