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His 标记 抗体 (FITC)

ABIN181860 产品详细信息, 供应商: Log in to see
抗原
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
适用
标记
616
18
3
1
宿主
小鼠
397
178
27
23
4
4
3
1
克隆类型 (克隆位点)
单克隆 ()
标记
FITC
58
44
34
15
13
11
9
8
8
7
7
7
7
7
7
7
6
6
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
应用范围
Flow Cytometry (FACS), Immunofluorescence (IF)
529
372
160
105
76
75
66
26
12
11
9
7
7
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
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Available images

免疫原 PAX6 transcription factor linked to histidine tag. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
序列 HHHHHH
克隆位点 AD1-1-10
亚型 IgG1
纯化方法 Affinity Chromatography on Protein G
别名 6xHistidine Epitope Tag
物质类 Tag
背景 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.
应用备注 Flow Cytometry: Use 10 μL of 1/10 diluted antibody to label 1x10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
浓度 0.1 mg/mL
缓冲液 PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
厂商提供的图像
Flow Cytometry (FACS) image for anti-His 标记 抗体 (FITC) (ABIN181860) Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blo...
背景 Diefenbacher, Thorsteinsdottir, Spang: "The Dsl1 tethering complex actively participates in soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor (SNARE) complex assembly at the endoplasmic reticulum in Saccharomyces cerevisiae." in: The Journal of biological chemistry, Vol. 286, Issue 28, pp. 25027-38, 2011 (PubMed).

Alvarez, López-Pacheco, Aguilar-Yañez, Portillo-Lara, Mendoza-Ochoa, García-Echauri, Freiden, Schultz-Cherry, Zertuche-Guerra, Bulnes-Abundis, Salgado-Gallegos, Elizondo-Montemayor, Hernández-Torre: "Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method." in: PLoS ONE, Vol. 5, Issue 4, pp. e10176, 2010 (PubMed).

Gunnarsen, Lunde, Kristiansen, Bogen, Sandlie, Løset: "Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA." in: BMC biotechnology, Vol. 10, pp. 8, 2010 (PubMed).

Wrighton, Lin, Yu, Feng: "Transforming Growth Factor {beta} Can Stimulate Smad1 Phosphorylation Independently of Bone Morphogenic Protein Receptors." in: The Journal of biological chemistry, Vol. 284, Issue 15, pp. 9755-63, 2009 (PubMed).

Bahi, Dreyer: "Overexpression of plasminogen activators in the nucleus accumbens enhances cocaine-, amphetamine- and morphine-induced reward and behavioral sensitization." in: Genes, brain, and behavior, Vol. 7, Issue 2, pp. 244-56, 2008 (PubMed).

Hwang, Duvall, Tomlinson, Boackle et al.: "Highly specific inhibition of C1q globular-head binding to human IgG: a novel approach to control and regulate the classical complement pathway using an engineered single chain antibody variable ..." in: Molecular immunology, Vol. 45, Issue 9, pp. 2570-80, 2008 (PubMed).

Bahi, Kusnecov, Dreyer: "The role of tissue-type plasminogen activator system in amphetamine-induced conditional place preference extinction and reinstatement." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 33, Issue 11, pp. 2726-34, 2008 (PubMed).

Manno: "Measurement of the digit lengths and the anogenital distance in mice." in: Physiology & behavior, Vol. 93, Issue 1-2, pp. 364-8, 2008 (PubMed).

Hoffmann, Schellack, Textor, Konold, Schmitz, Cerwenka, Pflanz, Watzl: "Identification of CLEC12B, an inhibitory receptor on myeloid cells." in: The Journal of biological chemistry, Vol. 282, Issue 31, pp. 22370-5, 2007 (PubMed).

Zheng, Kohler, Chen, Weber, Khan, Johnson, Orentas: "Serum from mice immunized in the context of Treg inhibition identifies DEK as a neuroblastoma tumor antigen." in: BMC immunology, Vol. 8, pp. 4, 2007 (PubMed).

Els Conrath, Lauwereys, Wyns, Muyldermans: "Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7346-50, 2001 (PubMed).

Suen, Wu, Chen, Wu, Chiang: "Characterization of self-T-cell response and antigenic determinants of U1A protein with bone marrow-derived dendritic cells in NZB x NZW F1 mice." in: Immunology, Vol. 103, Issue 3, pp. 301-9, 2001 (PubMed).