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GFP 抗体 (Green Fluorescent Protein) (AA 183-191)

Details for Product anti-GFP Antibody No. ABIN1742292, 供应商: Log in to see
抗原
  • green fluorescent protein
  • gfp
抗原表位
AA 183-191
42
31
27
18
5
4
3
3
2
1
1
1
1
1
适用
Aequorea victoria
639
5
5
2
2
2
1
1
宿主
小鼠
327
171
80
41
16
9
8
2
克隆类型 (克隆位点)
单克隆 ()
标记
This GFP antibody is un-conjugated
32
30
27
14
12
11
11
8
8
8
7
7
6
5
5
5
5
5
5
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
应用范围
Western Blotting (WB)
522
338
189
144
141
120
96
72
58
26
15
13
13
8
8
6
6
5
4
4
3
3
3
3
3
2
2
1
1
1
1
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Available images

免疫原 Recombinant full length GFP, glutaraldehyde fixed. Epitope QQNTPIGDG (aa 183-191)
克隆位点 101G4
亚型 IgG2b
特异性 Specific for GFP of jelly-fish.
纯化方法 purified IgG. Azide was added before lyophilization.
别名 GFP
研究领域 Tags/Labels
应用备注 WB: 1 : 1000 up to 1 : 10 000 (AP staining)
IP: no (methanol fixated material only)
IHC: not tested yet
说明

ABIN1742292 is recommended for IP and ICC.

限制 仅限研究用
状态 Lyophilized
溶解方式 For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
缓冲液 PBS, 0.02% sodium azide
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
储存条件 -20 °C
储存方法 Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
厂商提供的图像
Western Blotting (WB) image for anti-GFP 抗体 (Green Fluorescent Protein) (AA 183-191) (ABIN1742292) dilution: 1 : 1000, sample: recombinant protein
有引用在: Koestler, Steffen, Nemethova, Winterhoff, Luo, Holleboom, Krupp, Jacob, Vinzenz, Schur, Schlüter, Gunning, Winkler, Schmeiser, Faix, Stradal, Small, Rottner: "Arp2/3 complex is essential for actin network treadmilling as well as for targeting of capping protein and cofilin." in: Molecular biology of the cell, Vol. 24, Issue 18, pp. 2861-75, 2013 (PubMed).

Oelkers, Vinzenz, Nemethova, Jacob, Lai, Block, Szczodrak, Kerkhoff, Backert, Schlüter, Stradal, Small, Koestler, Rottner: "Microtubules as platforms for assaying actin polymerization in vivo." in: PLoS ONE, Vol. 6, Issue 5, pp. e19931, 2011 (PubMed).

Kaddai, Gonzalez, Keslair, Grémeaux, Bonnafous, Gugenheim, Tran, Gual, Le Marchand-Brustel, Cormont: "Rab4b is a small GTPase involved in the control of the glucose transporter GLUT4 localization in adipocyte." in: PLoS ONE, Vol. 4, Issue 4, pp. e5257, 2009 (PubMed).