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GFP 抗体 (Biotin)

Name: GFP 抗体 (Biotin)
SKU: ABIN117892
Availability: OutOfStock

GFP 适用: Aequorea victoria WB, IHC (fro), EIA 宿主: 小鼠 Monoclonal 9F9-F9 Biotin

产品编号 ABIN117892

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
适用
175

19

15

15

12

7

5

4

2

1

1

1

1
Aequorea victoria
宿主
74

73

35

11

9

6

2

1
小鼠
Compatible Secondaries
克隆类型
121

85
单克隆
标记
104

14

12

9

6

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to Biotin
应用范围
173

87

55

42

33

31

29

21

16

14

14

14

12

6

4

4

3

3

2

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

特异性

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Mouse Serum. Reactivity is observed against wild type and recombinant forms of GFP. Reactivity is observed against recombinant Green Fluorescent Protein (recombinant GFP from Aequorea victoria) by both Western blot and ELISA. No reaction is seen against RFP.

产品特性

Molar Ratio: 10-20 BAC molecules per Mouse IgG molecule.

纯化方法

Protein A chromatography.

免疫原

Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria

克隆位点

9F9-F9

亚型

IgG2a

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应用备注

Monclonal anti-GFP is designed to detect native GFP and GFP-containing recombinantproteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for thedirect binding of antigen. Biotin conjugated monoclonal anti-GFP used in a sandwich ELISAis well suited to titrate GFP in solution using this antibody in combination with polyclonal

限制

仅限研究用
溶解方式

Restore with 1.0 mL of deionized water (or equivalent).

浓度

1.0 mg/mL (by UV absorbance at 280 nm)

缓冲液

0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide, 10 mg/mL BSA (IgG and Protease free)

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing. Dilute only prior to immediate use

储存条件

4 °C/-20 °C

储存方法

Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

别名

green fluorescent protein antibody, gfp antibody

物质类

Viral Protein

背景

Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein

基因ID

6100

UniProt

P42212