DYKDDDDK 标记 抗体

ABIN1043869 产品详细信息, 供应商: Log in to see
抗原
适用
标记
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宿主

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克隆类型
多克隆
标记
非结合性
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应用范围
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
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免疫原 This antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the carboxy terminal end to facilitate coupling. This antibody reacts with DYKDDDDK conjugated proteins.
Immunogen Type: Peptide
序列 DYKDDDDK
亚型 IgG
特异性 This affinity purified antibody is directed against the DYKDDDDK motif and is useful in determining its presence in various assays. This polyclonal anti-DYKDDDDK™ tag antibody detects over-expressed proteins containing the DYKDDDDK epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
产品特性 Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.
过滤 Sterile filtered
物质类 Tag
背景 Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.
应用备注 This antibody is optimally suited for monitoring the expression of DYKDDDDK tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the DYKDDDDK epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and DYKDDDDKä containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. Now the most commonly used hydrophilic octapeptide is DYKDDDDK. polyclonal antibody to detect DYKDDDDK conjugated proteins binds DYKDDDDK containing fusion proteins with greater affinity than the widely used monoclonal M1, M2 and M5 clones, and shows greater sensitivity in most assays. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.
限制 仅限研究用
状态 Liquid
浓度 1.04 mg/mL
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
有效期 12 months
厂商提供的图像
 image for anti-DYKDDDDK 标记 抗体 (ABIN1043869) anti-DYKDDDDK Tag antibody
有引用在: Gassen, Hartmann, Zschocke, Stepan, Hafner, Zellner, Kirmeier, Kollmannsberger, Wagner, Dedic, Balsevich, Deussing, Kloiber, Lucae, Holsboer, Eder, Uhr, Ising, Schmidt, Rein: "Association of FKBP51 with Priming of Autophagy Pathways and Mediation of Antidepressant Treatment Response: Evidence in Cells, Mice, and Humans." in: PLoS medicine, Vol. 11, Issue 11, pp. e1001755, 2014 (PubMed).

Chubet, Brizzard: "Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells." in: BioTechniques, Vol. 20, Issue 1, pp. 136-41, 1996 (PubMed).