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GFP 抗体 (Green Fluorescent Protein) (AA 246) (HRP)

Details for Product anti-GFP Antibody No. ABIN100089, 供应商: Log in to see
抗原
  • green fluorescent protein
  • gfp
抗原表位
AA 246
42
31
27
17
5
4
3
3
2
1
1
1
1
1
1
适用
Aequorea victoria
639
5
5
2
2
2
1
1
宿主
山羊
328
171
79
41
16
9
8
2
克隆类型
多克隆
标记
This GFP antibody is conjugated to HRP
32
29
27
14
12
11
11
8
8
8
7
7
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5
5
5
5
5
5
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
应用范围
Fluorescence Microscopy (FM), ELISA, Western Blotting (WB)
522
337
189
144
141
120
96
72
58
26
15
13
12
8
8
6
6
5
4
4
3
3
3
3
3
2
2
1
1
1
1
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Available images

免疫原 The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence derived from the jellyfish Aequorea victoria.
Immunogentype:Recombinant
亚型 IgG
产品特性 Concentration Definition: by UV absorbance at 280 nm
别名 GFP
背景 HRP Anti-GFP is ideal for western blotting, ELISA and Immunohistochemistry. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Synonyms: HRP, GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.
研究领域 Tags/Labels
应用备注 Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
限制 仅限研究用
状态 Lyophilized
溶解方式 Restore with deionized water (or equivalent)
浓度 1.0 mg/mL
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液 Gentamicin sulfate
注意事项 Do NOT add Sodium Azide!
储存条件 4 °C
厂商提供的图像
Western Blotting (WB) image for anti-GFP 抗体 (Green Fluorescent Protein) (AA 246) (HRP) (ABIN100089) Western Blot of Anti-GFP Antibody Peroxidase Conjugate. Lane 1: 50ng of GFP. Lane 2: ...
有引用在: Chatain, Ziegler, Fahrenkamp, Jost, Moriggl, Schmitz-Van de Leur, Müller-Newen: "Src family kinases mediate cytoplasmic retention of activated STAT5 in BCR-ABL-positive cells." in: Oncogene, 2012 (PubMed).