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Normal 驴 Serum

BI, ELISA, ICC, IF, IHC 宿主: 驴
产品编号 ABIN7232252
发货至: 中国
  • 抗原
    Normal Serum
    宿主
    应用范围
    BioImaging (BI), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
    原理
    Normal Donkey Serum
    纯化方法
    Lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2
    纯度
    Whole serum
  • 应用备注
    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-p (1:10-20), IF/ICC (1:10-20) and ELISA (1:100).
    说明

    Blocking Serum

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    10 mM sodium phosphate, 0.15 M sodium chloride with 0.05 % sodium azide as preservative, pH 7.2
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store at 2-8°C or aliquot and store at or below -20°C for long-term storage. Avoid repeated freezing and thawing.
  • 抗原
    Normal Serum
    背景
    High quality Normal donkey serum processed from non-immunized normal adult donkeys is used as a blocking agent or as a negative control for immunoassays.,Normal donkey serums, which are from non-immuned donkey hosts, are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide. Normal serum diluted to 5 % -20 % (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific binding, or control for most immunoassay applications. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.,Serum
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