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山羊 anti-兔 IgG (Heavy & Light Chain) Antibody - Preadsorbed

ELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
产品编号 ABIN965369
发货至: 中国
  • 抗原 See all IgG products
    IgG
    片段
    Fab fragment
    抗原表位
    • 2355
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    Heavy & Light Chain
    适用
    • 697
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    宿主
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    山羊
    克隆类型
    • 3353
    • 193
    • 1
    多克隆
    应用范围
    • 1866
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Rabbit IgG whole molecule

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    ELISA Dilution: 1:4,000 - 1:20,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature.
    储存条件
    4 °C
    储存方法
    Store vial at 4 °C prior to opening. This product is stable at 4 °C as an undiluted liquid.
    有效期
    12 months
  • Dembla, Kesharwani, Natarajan, Fecher-Trost, Fairless, Williams, Flockerzi, Diem, Schwarz, Schmitz: "Early auto-immune targeting of photoreceptor ribbon synapses in mouse models of multiple sclerosis." in: EMBO molecular medicine, Vol. 10, Issue 11, (2019) (PubMed).

  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: Goat Fab Anti-Rabbit IgG Antibody, Goat Fab Fragment Anti-Rabbit IgG Antibody

    Background: Fab Anti-Rabbit IgG (H&L) Antibody generated in goat detects immunoglobulin g from rabbit, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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