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兔 anti-小鼠 IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Biotin Fab fragment
产品编号 ABIN965366
发货至: 中国
  • 抗原 See all IgG products
    IgG
    片段
    Fab fragment
    抗原表位
    • 2355
    • 502
    • 411
    • 188
    • 130
    • 124
    • 94
    • 29
    • 28
    • 28
    • 17
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    适用
    • 696
    • 680
    • 568
    • 452
    • 368
    • 239
    • 200
    • 148
    • 140
    • 132
    • 122
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    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    小鼠
    宿主
    • 1728
    • 1485
    • 537
    • 216
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    克隆类型
    • 3354
    • 193
    • 1
    多克隆
    标记
    • 587
    • 410
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    • 368
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    • 228
    • 120
    • 99
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    • 10
    Biotin
    应用范围
    • 1867
    • 1788
    • 1785
    • 1535
    • 988
    • 934
    • 913
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
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    • 50
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    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Rabbit Serum.
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Mouse IgG whole molecule

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 500 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    0.5 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
    储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: Rabbit Fab Anti-Mouse IgG Biotin Conjugated Antibody, Rabbit Fab Fragment Anti-Mouse IgG Antibody Biotin Conjugation

    Background: Fab Anti-Mouse IgG (H&L) Biotin Antibody generated in rabbit detects Mouse IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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