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兔 anti-山羊 IgG Antibody (DyLight 649)

FACS, IF, ICC Polyclonal IgG DyLight 649
产品编号 ABIN7205190
发货至: 中国
  • 抗原 See all IgG products
    IgG
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    DyLight 649
    应用范围
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    Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)
    原理
    Dylight 649, Rabbit Anti-Goat IgG
    特异性
    The antibody reacts with whole molecule goat IgG. It also reacts with heavy chains of goat IgG, and light chains of all other goat immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.
    纯化方法
    Affinity purified using solid phase Goat IgG (H&L) with finally > 95% purity based on SDS-PAGE
    免疫原
    Goat IgG whole molecule
    亚型
    IgG
  • 应用备注
    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications.
    说明

    DyLight fluorescent dyes are a new family of dyes with improved brightness and photostability. They are better than or comparable to the best fluorescent dyes from other companies. The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye, antibody activity, specificity, and cross-reactivity, and the optimal moles of dye per antibody. A molar saturation curve vs fluorescence intensity, antibody activity, background level, and/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background. DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    Liquid in PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative, 1 % BSA as stablizer and 50 % Glycerol.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景
    Dylight 649 conjugated Rabbit Anti-Goat IgG (H+L) secondary antibody,Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.,Rabbit Anti-Goat IgG
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