兔 anti-小鼠 IgG1 (Chain gamma 1) Antibody (Cy2) - Preadsorbed
-
- 抗原 See all IgG1 products
- IgG1
-
抗原表位
- Chain gamma 1
-
适用
- 小鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- Cy2
-
应用范围
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- 产品特性
-
Synonyms: Rabbit Anti Mouse IgG1 (Gamma 1 chain) Antibody Cy2 Conjugated, Rabbit Anti-Mouse IgG1 Antibody Cy2 Conjugated
Background: Anti-Mouse IgG1 Cy2 Antibody generated in rabbit detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
-
Immunogen: Mouse IgG1 heavy chain
- 亚型
- IgG
- Labeling Ratio
- 3.6
-
-
- 应用备注
-
Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- IgG1
- Abstract
- IgG1 产品
- 物质类
- Antibody
-