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兔 anti-马 IgG Antibody (Cy3) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG Cy3 F(ab')2 fragment
产品编号 ABIN6698832
发货至: 中国
  • 抗原 See all IgG products
    IgG
    片段
    F(ab')2 fragment
    适用
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    多克隆
    标记
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    Cy3
    应用范围
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    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    产品特性

    Synonyms: Rabbit F(ab')2 Anti-Horse IgG Antibody CY3 Conjugation, Rabbit F(ab')2 Anti-Horse IgG CY3 Conjugated Antibody, Rabbit Fab2 Anti-Horse IgG CY3 Conjugated Antibody

    Background: F(ab')2 Anti-Horse IgG Cy3 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Horse IgG whole molecule

    亚型
    IgG
    Labeling Ratio
    5.1
  • 应用备注

    Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 250 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
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