兔 anti-小鼠 Immunoglobulin kappa Chain Complex (Igk) (Light Chain) Antibody (HRP) - Preadsorbed
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- 抗原 See all Igk products
- Igk (Immunoglobulin kappa Chain Complex (Igk))
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抗原表位
- Light Chain
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- HRP
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- 交叉反应
- 小鼠
- 产品特性
- Anti-Mouse kappa light chain antibody generated in rabbit detects specifically Mouse kappa light chain. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Anti-Mouse kappa light chain was produced by repeated immunization with Mouse kappa light chain in rabbit.
Immunogen Type: Native Protein
- 亚型
- IgG
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Rat anti-Mouse Immunoglobulin kappa Chain Complex (Igk) antibody
Igk FACS, ELISA Monoclonal 187-1 IgG1 unconjugated
Rat anti-Mouse Immunoglobulin kappa Chain Complex (Igk) antibody (FITC)Igk FACS, ELISA Monoclonal H139-52-1 IgG1 FITC
Goat anti-Mouse Immunoglobulin kappa Chain Complex (Igk) antibodyIgk FACS, ELISA Polyclonal IgG unconjugated F(ab')2 fragment
Rat anti-Mouse Immunoglobulin kappa Chain Complex (Igk) antibody (Biotin)Igk FACS, ELISA Monoclonal 187-1 IgG1 Biotin
Rat anti-Mouse Immunoglobulin kappa Chain Complex (Igk) antibody (PE)Igk FACS, ELISA Monoclonal 187-1 IgG1 PE
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- 应用备注
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Immunohistochemistry Dilution: 1:500 - 1:2,500
Application Note: Anti-Mouse kappa light chain antibody generated in rabbit detects specifically Mouse kappa light chain. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
ELISA Dilution: 1:10,000 - 50,000
Western Blot Dilution: 1:2,000 - 1:5,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Thimerosal
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Igk (Immunoglobulin kappa Chain Complex (Igk))
- 别名
- kappa ) (Igk 产品)
- 背景
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Synonyms: Rabbit Anti-Mouse κ (kappa chain) Antibody peroxidase Conjugated, Rabbit Anti-Mouse kappa Antibody HRP Conjugation
Background: Anti-Mouse κ (kappa chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse kappa light chain. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). The light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG kappa is conjugated to peroxidase.
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