山羊 anti-大鼠 IgA, IgG, IgM (Heavy & Light Chain) Antibody (HRP) - Preadsorbed
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- 抗原 See all IgA, IgG, IgM products
- IgA, IgG, IgM
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抗原表位
- Heavy & Light Chain
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适用
- 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- HRP
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 产品特性
- Rat IgG IgA IgM (H&L) Antibody detects immunoglobulin G, A, and M from Rat. Immunoglobulin G binds to antigens and can neutralize or opsinize targts, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunogobulin molecules linked through their F(c) domain by the J chain.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Rat IgG, IgA and IgM whole molecules
- 亚型
- IgG
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- 应用备注
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Immunohistochemistry Dilution: 1:500-1:1,500
Application Note: Anti-Rat IgG IgA IgM Antibody is suitable for immunoassays where reactivity to different classes of rat immunoglobulins is desired. Antibody has been tested by ELISA, western blot immunoblot, and immunohistochemistry. Optimal concentrations in immunoassays should be determined by the researcher.
ELISA Dilution: 1:100,000
Western Blot Dilution: 1:500-1:3,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 2.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Thimerosal
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgA, IgG, IgM
- 别名
- IgG IgA IgM (IgA, IgG, IgM 产品)
- 物质类
- Antibody
- 背景
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Synonyms: goat anti-Rat IgG IgA IgM Antibody peroxidase conjugation, Goat Anti-rat IgGAM HRP conjugated antibody
Background: Rat IgG IgA IgM (H&L) Antibody detects immunoglobulin G, A, and M from Rat. Immunoglobulin G binds to antigens and can neutralize or opsinize targts, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunogobulin molecules linked through their F(c) domain by the J chain.
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