山羊 anti-人 IgG (Heavy Chain) Antibody - Preadsorbed
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- 抗原 See all IgG products
- IgG
- 片段
- F(ab')2 fragment
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抗原表位
- Heavy Chain
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 产品特性
- F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Human IgG gamma heavy chain
- 亚型
- IgG
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Goat anti-Human IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Goat anti-Human IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin
Mouse anti-Human IgG (Fab Region) antibodyELISA, WB, FACS Monoclonal 4A11 IgG2b unconjugated
Goat anti-Human IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Goat anti-Human IgG (F(ab')2 Region) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated F(ab')2 fragment
Mouse anti-Human IgG (Fc Region) antibody (HRP)ELISA, IHC, WB Monoclonal JDC-10 IgG1 HRP
Goat anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Rabbit anti-Human IgG (Fc Region) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Mouse anti-Human IgG (Heavy & Light Chain) antibodyELISA, WB Monoclonal 4D2D9G8-4A10 IgG1 unconjugated
Goat anti-Human IgG (Fc Region) antibody (HRP)ELISA, WB Polyclonal HRP F(ab')2 fragment
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.36 mg/mL
- 缓冲液
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Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: Goat F(ab')2 Anti-Human IgG (gamma chain) Antibody, Goat F(ab')2 Anti-Human IgG gamma Antibody
Background: F(ab')2 Anti-Human IgG (gamma chain) Antibody generated in goat detects human immunoglobulin g (gamma chain). Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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