ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
原理
Rabbit anti-pig IgG recognizes pig IgM mu heavy chain. This secondary antibody was purified using delipidation, salt fractionation and ion exchange chromatography. The antibody is conjugated with Peroxidase.
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Swine IgM and Swine Serum. No reaction was observed against Swine IgG.
交叉反应
Pig
交叉反应 (详细)
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Swine IgM and Swine Serum. No reaction was observed against Swine IgG.
ELISA (1:10000-1:50000) Western Blot (1:1000-1:10000) Immunohistochemistry (1:500-1:2500) The optimal working dilution should be determined by the end user.
限制
仅限研究用
状态
Lyophilized
缓冲液
Lyophilized from 0.02 M KH2PO4, 150 mM NaCl, pH 7.2 (0.01 % gentamicin sulfate).
储存液
Gentamicin sulfate
注意事项
This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Store at 4°C on dry atmosphere. After reconstitution with 0.1 mL of deionized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.