兔 anti-绵羊 IgM (Heavy & Light Chain) Antibody
WB, IF, ICC, DB, EIA
Polyclonal
IgG
unconjugated
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- 抗原 See all IgM products
- IgM
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抗原表位
- Heavy & Light Chain
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适用
- 绵羊
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宿主
- 兔
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克隆类型
- 多克隆
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Dot Blot (DB), Enzyme Immunoassay (EIA)
- 纯化方法
- Hyperimmune antisera with strong activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
- 免疫原
- Purified normal IgM isolated from pooled sheep serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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- 应用备注
- ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemistry Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Precipitin titre : 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
- 限制
- 仅限研究用
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- 缓冲液
- PBS, pH 7.2 Preservatives: None, as it may interfere with the antibody activity. Stabilizers: None
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
- 有效期
- 12 months
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
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