山羊 anti-豚鼠 IgG (Heavy & Light Chain) Antibody (HRP)
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 豚鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- HRP
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 预测反应
- guinea pig IgG heavy and light chains (H&L) of all guinea pig immunoglobulins
- 产品特性
- Goat anti-guinea pig IgG is a secondary antibody conjugated to HRP which binds to all guinea pig IgGs in immunological assays.
- 纯化方法
- Affinity purification
- 免疫原
- Purified Guinea pig IgG, whole molecule
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- 应用备注
- 1: 10 000 -1: 50 000 (ELISA), (WB), 1: 500 -1: 5000 (IHC)
- 说明
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This antibody reacts with the heavey chaings on guinea pig IgG and with the light chains on all guinea pig immunoglobulins based on immunoelectrophoresis.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
- 缓冲液
- 10 mM Sodium Phosphate, 0.15 M SodiumChloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative.
- 储存液
- Kathon CG
- 注意事项
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Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. - 储存条件
- 4 °C
- 储存方法
- Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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