山羊 anti-人 IgG (Chain kappa), (Chain lambda), (Fab Region), (Free & Bound) Antibody (TRITC)
IF
Polyclonal
IgG
TRITC
F(ab')2 fragment
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- 抗原 See all IgG products
- IgG
- 片段
- F(ab')2 fragment
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抗原表位
- Chain kappa, Chain lambda, Fab Region, Free & Bound
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- TRITC
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应用范围
- Immunofluorescence (IF)
- 特异性
- The antiserum is reacting with to the Fab subunits of the IgG, IgA, IgM and other Ig classes of both light chain type, with their Fab or F(ab')2 subunits and with free immunoglobulin light chains of kappa and lambda type.
- 产品特性
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Tetramethylrhodamine isothiocyanate-conjugated F(ab')2 fragments IgG antibodies of poly-clonal goat antiserum to human Fab IgG
Fluorochrome/IgG protein molar ratio (F/P) is approximately 2.2. - 纯化方法
- No adsorption required. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
- 免疫原
- Purified Fab prepared from normal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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- 应用备注
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Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
- 浓度
- 10 mg/mL
- 缓冲液
- PBS, pH 7.2
- 储存液
- Without preservative
- 注意事项
- Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen , and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifu-gation. It will not affect the performance of the immunoconjugate.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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