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小鼠 anti-人 IgG3 (Allotype G3m(U)) Antibody (FITC)

ELISA, ICC, IF, IHC (fro) Monoclonal NI 86 (HP 6080) IgG1 FITC
产品编号 ABIN457899
发货至: 中国
  • 抗原 See all IgG3 products
    IgG3
    抗原表位
    • 38
    • 15
    • 6
    • 4
    • 4
    • 1
    • 1
    • 1
    Allotype G3m(U)
    适用
    • 71
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    宿主
    • 37
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    小鼠
    克隆类型
    • 64
    • 26
    单克隆
    标记
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    • 1
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    FITC
    应用范围
    • 50
    • 41
    • 36
    • 32
    • 26
    • 21
    • 10
    • 7
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    • 5
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    ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
    特异性
    Fluorescein isothiocyanate-conjugated purified monoclonal Mouse antibody to Human IgG3m(u), allotype specific.
    交叉反应 (详细)
    The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.
    产品特性
    The reactivity of the antiserum is restricted to an allotype specific determinant on the IgG3m(u) molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG3m(u) in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG3m(u), and immunoblotting using a peroxidase labelled monoclonal antibody against FITC. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50, in ELISA from 1:200 upwards, in Western blotting from 1:400 upwards.
    纯化方法
    Purified
    免疫原
    Highly purified monoclonal IgG3m(u) isolated from human serum.
    克隆位点
    NI 86 (HP 6080)
    亚型
    IgG1
  • 应用备注
    ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.
    缓冲液
    Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
    储存液
    Without preservative
    储存条件
    RT,4 °C,-20 °C
    储存方法
    The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.
  • 抗原
    IgG3
    Abstract
    IgG3 产品
    物质类
    Antibody
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