小鼠 anti-人 IgG3 (Allotype G3m(U)) Antibody (Biotin)
-
- 抗原 See all IgG3 products
- IgG3
-
抗原表位
- Allotype G3m(U)
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- Biotin
-
应用范围
- ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- Biotin-conjugated purified monoclonal Mouse antibody to Human IgG3m(u), allotype specific.
- 交叉反应 （详细）
- The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.
- 产品特性
- The reactivity of the antiserum is restricted to the allotype specific determinant on the IgG3 Molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG3m(u) in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:100 to 1:500, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards.
- 纯化方法
- Purified
- 免疫原
- Highly purified monoclonal IgG3 isolated from human serum.
- 克隆位点
- NI 86 (HP 6080)
- 亚型
- IgG1
-
Mouse anti-Human IgG3 (Hinge Region) antibody
ELISA, WB, IHC, ICC Monoclonal HP6050 IgG1 unconjugated
Mouse anti-Human IgG3 (Hinge Region) antibody (PE)ELISA, IHC, ICC Monoclonal HP6050 IgG1 PE
Mouse anti-Human IgG3 (Hinge Region) antibody (Alkaline Phosphatase (AP))ELISA, WB, IHC Monoclonal HP6050 IgG1 Alkaline Phosphatase (AP)
Mouse anti-Human IgG3 (Hinge Region) antibody (Biotin)ELISA, WB, IHC, ICC Monoclonal HP6050 IgG1 Biotin
Mouse anti-Human IgG3 (Hinge Region) antibody (FITC)ELISA, IHC, ICC Monoclonal HP6050 IgG1 FITC
Mouse anti-Human IgG3 (Hinge Region) antibody (HRP)ELISA, WB, IHC Monoclonal HP6050 IgG1 HRP
-
- 应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.
- 缓冲液
- Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
- 储存液
- Without preservative
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.
-
- 抗原
- IgG3
- Abstract
- IgG3 产品
- 物质类
- Antibody
-
