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小鼠 anti-人 IgG1 (Fc Region) Antibody (Biotin)

Name: 小鼠 anti-人 IgG1 (Fc Region) Antibody (Biotin)
Brand: antibodies-online
SKU: ABIN457885
Availability: OutOfStock

ELISA, WB Monoclonal NI 132 (HP 6186) IgG1 kappa Biotin

产品编号 ABIN457885

发货至: 中国

Datasheet as PDF

抗原 See all IgG1 products

IgG1
抗原表位
42

25

17

6

5

3

1

1

1

1

1

1
Fc Region
适用
85

25

23

7

7

5

5

4

3

2

1
人
宿主
64

39

35

10

9

6

1
小鼠
克隆类型
97

54
单克隆
标记
45

18

18

15

11

8

7

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Biotin
应用范围
76

57

51

42

32

21

18

13

11

10

10

9

6

4

3

2

2

2
ELISA, Western Blotting (WB)

特异性

The reactivity of the antiserum is restricted to the subclass specific determinant on the Fc portion of the IgG1 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunoperoxidase staining of cytoplasmic Ig.

产品特性

Biotin-conjugated purified monoclonal mouse antibody to human IgG1, subclass specific
Biotin/IgG protein molar ratio (B/P) approximately 2.4.

纯化方法

Purified

免疫原

Highly purified monoclonal IgG1 isolated from pooled human serum.

克隆位点

NI 132 (HP 6186)

亚型

IgG1 kappa
Mouse anti-Human IgG1 (Hinge Region) antibody
ELISA Monoclonal 4E3 IgG1 unconjugated

Mouse anti-Human IgG1 (Hinge Region) antibody (HRP)
ELISA Monoclonal 4E3 IgG1 HRP

Rabbit anti-Human IgG1 (AA 211-260) antibody
WB, IHC (p) Polyclonal unconjugated

Mouse anti-Human IgG1 (Hinge Region) antibody (FITC)
ELISA Monoclonal 4E3 IgG1 FITC

Mouse anti-Human IgG1 (Hinge Region) antibody (PE)
ELISA Monoclonal 4E3 IgG1 PE

Rabbit anti-Human IgG1 (Internal Region) antibody
ELISA, WB, IHC Polyclonal IgG unconjugated

Mouse anti-Human IgG1 (Fc Region) antibody
ELISA, FLISA Monoclonal HP6001 IgG2b unconjugated

Mouse anti-Human IgG1 (Hinge Region) antibody (Alkaline Phosphatase (AP))
ELISA Monoclonal 4E3 IgG1 Alkaline Phosphatase (AP)

Mouse anti-Human IgG1 (Fc Region) antibody (Alkaline Phosphatase (AP))
ELISA, FLISA Monoclonal HP6001 IgG2b Alkaline Phosphatase (AP)

Mouse anti-Human IgG1 (Hinge Region) antibody (Biotin)
ELISA Monoclonal 4E3 IgG1 Biotin

应用备注

To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmicIgG1, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:40, in ELISA from 1:100 to 1:2000, in Western blotting from 1:200 to 1:4000. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

浓度

0.4 mg/mL

缓冲液

Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH 7.2).

储存液

Without preservative

注意事项

Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutionsshould be stored at +4 °C, not refrozen, and preferably used the same day.

储存条件

4 °C/-20 °C

储存方法

The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
抗原

IgG1

Abstract

IgG1 产品

物质类

Antibody