小鼠 anti-人 IgA2 (Allotype IgA2(m)2) Antibody (HRP)
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- 抗原 See all IgA2 products
- IgA2
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抗原表位
- Allotype IgA2(m)2
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- HRP
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应用范围
- ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)
- 特异性
- Horseradish peroxidase conjugated purified monoclonal Mouse antibody to Human IgA2(m)2, allotype specific
- 交叉反应 （详细）
- The antibody does not react with any other component of the Human immunoglobulin system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.
- 产品特性
- The reactivity of the this monoclonal antibody is restricted to polyclonal and monoclonal IgA2(m)2, as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, latex agglutination assay and histochemistry immunoprecipitation and direct immunoperoxidase staining. (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148) To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50, in ELISA from 1:60 upwards, in Western blotting from 1:120 upwards.
- 纯化方法
- Purified
- 免疫原
- Highly purified monoclonal IgA2(m)2 isolated from human serum.
- 克隆位点
- NI 194-3 (A89-040)
- 亚型
- IgG1
- Labeling Ratio
- Peroxidase/IgG protein molar ratio (E/P) approximately 1.7.
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Mouse anti-Human IgA2 (Fc Region) antibody
ELISA Monoclonal A9604D2 IgG1 unconjugated
Mouse anti-Human IgA2 antibody (PE)ELISA Monoclonal A9604D2 IgG1 PE
Mouse anti-Human IgA2 (Fc Region) antibody (HRP)ELISA, WB, IHC Monoclonal A9604D2 IgG1 HRP
Mouse anti-Human IgA2 (Fc Region) antibody (Biotin)ELISA Monoclonal A9604D2 IgG1 Biotin
Mouse anti-Human IgA2 antibody (FITC)ELISA Monoclonal A9604D2 IgG1 FITC
Mouse anti-Human IgA2 (Fc Region) antibody (Alkaline Phosphatase (AP))ELISA, WB, IHC Monoclonal A9604D2 IgG1 Alkaline Phosphatase (AP)
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- 应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration 0.4 mg/mL. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. No foreign proteins added. Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.
- 缓冲液
- Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration 0.4 mg/mL.
- 储存液
- Without preservative
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.
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- 抗原
- IgA2
- 别名
- IgA22 (IgA2 产品)
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