The reactivity of the antiserum is directed to the Fc and Fab subunits of the major immunoglobulins.
产品特性
Total protein and IgG concentration in the antiserum are comparable to those of pooled sheep serum. Antibody titre: Precipitin titre not less than 1:32 when tested against normal guinea pig serum in agar block titration.
纯化方法
Delipidated, heat inactivated, lyophilized stable whole serum Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity with other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
免疫原
Purified normal Ig fractions isolated from pooled guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
亚型
IgG
应用备注
In immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins, as secondary antibody to precipitate the immunoglobulin in normal guinea pig serum.
限制
仅限研究用
状态
Lyophilized
溶解方式
It is reconstituted by adding 1 mL sterile distilled water.
缓冲液
No foreign proteins added.
储存液
Without preservative
注意事项
Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, a nd preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
储存条件
4 °C/-20 °C
储存方法
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.