山羊 anti-猴 IgM (Fc Region) Antibody (Biotin)
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- 抗原 See all IgM products
- IgM
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抗原表位
- Fc Region
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适用
- 猴
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Biotin
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应用范围
- ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunodiffusion (ID), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)
- 特异性
- Monkey IgM, Fc specific.
- 纯化方法
- Purified IgG
- 免疫原
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Purified IgM isolated from Rhesus monkey serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Type of Immunogen: Purified protein
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Goat anti-Monkey IgM (Chain mu) antibody (Biotin) - Preadsorbed
ELISA, IHC, WB Polyclonal IgM Biotin
Goat anti-Monkey IgM (Chain mu) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgM unconjugated
Goat anti-Monkey IgM (Chain mu) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgM HRP
Goat anti-Monkey IgM (Chain mu) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
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- 应用备注
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Approved: DB, ELISA, ICC, ID, IHC, IHC-P
Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with IgG, IgA and IgG/Fab or any non-Ig protein in monkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates. to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen using a reference antibody of monkey origin known to be of the IgM isotype in the middle layer of the indirect test procedure. in non-isotopic assay methodology (e. g. ELISA) to measure IgM in monkey serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:3000. - 说明
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Target Species of Antibody: Monkey
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, pH 7.2. No preservative added.
- 储存液
- Without preservative
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- 抗原
- IgM
- 别名
- IgM, (IgM 产品)
- 物质类
- Antibody
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