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兔 anti-山羊 IgG Antibody (TRITC)

ELISA, IHC, IF, ICC, ID, IHC (fro) Polyclonal TRITC
产品编号 ABIN2623334
发货至: 中国
  • 抗原 See all IgG products
    IgG
    适用
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    TRITC
    应用范围
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    ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunodiffusion (ID), Immunohistochemistry (Frozen Sections) (IHC (fro))
    特异性
    Goat IgG, heavy and light chains.
    纯化方法
    Purified IgG
    免疫原
    Purified normal IgG isolated from pooled goat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

    Type of Immunogen: Purified protein
  • 应用备注
    Approved: ELISA, ICC, ID, IF, IHC, IHC-Fr

    Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in goat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates. If two immunoconjugates with different labels but originating from the same host animal species are used for direct two-color staining, they can safely be mixed and used in a single step, without the danger of interaction between the two reagents. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.
    说明

    Target Species of Antibody: Goat

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Distilled water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS,  pH 7.2. No preservative added.
    储存液
    Without preservative
    注意事项
    Avoid repeat freeze-thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
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