猪 anti-人 IgE (Fc Region) Antibody (FITC)
ELISA, IHC, IF, ICC, IHC (fro)
Polyclonal
FITC
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- 抗原 See all IgE products
- IgE
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抗原表位
- Fc Region
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适用
- 人
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宿主
- 猪
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克隆类型
- 多克隆
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标记
- FITC
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应用范围
- ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Human IgE Fc specific.
- 纯化方法
- Purified IgG
- 免疫原
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Pools of purified homogenous IgE isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Type of Immunogen: Purified protein
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Mouse anti-Human IgE (Fc Region) antibody (Biotin)
ELISA Monoclonal HP6029 IgG2a Biotin
Mouse anti-Human IgE (Fc Region) antibodyELISA, WB, FACS, Func Monoclonal Le27-NBS01 IgG1 kappa unconjugated
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- 应用备注
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Approved: ELISA, ICC, IF, IHC, IHC-Fr
Usage: The reactivity of the antiserum is directed to the Fc subunit of the IgE molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against human serum, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgE used as immunogens. It does not react with IgG including all subclasses, IgG/Fab fragments, IgM and IgA or any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. The antiserum also react with membrane-bound IgE in peripheral blood cells as tested by immunofluorescence microscopy. In immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. to identify and measure IgE in human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80, depending on the method used. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, pH 7.2. No preservative added.
- 储存液
- Without preservative
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- 抗原
- IgE
- Abstract
- IgE 产品
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