兔 anti-猴 IgA (Fc Region) Antibody (FITC)
ELISA, IHC, IF, ICC, IHC (fro)
Polyclonal
FITC
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- 抗原 See all IgA products
- IgA
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抗原表位
- Fc Region
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适用
- 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- FITC
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应用范围
- ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Monkey IgA Fc specific.
- 纯化方法
- Purified IgG
- 免疫原
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Pools of purified IgA isolated from Rhesus monkey serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Type of Immunogen: Purified protein
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- 应用备注
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Approved: ELISA, ICC, IF, IHC, IHC-Fr
Usage: The reactivity of the antiserum is directed to the Fc subunit of the IgA molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against monkey serum, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgA used as immunogens. It does not react with IgG, IgG/Fab fragments and IgM or any non-Ig protein in monkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates. to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen using a reference antibody of monkey origin known to be of the IgA isotype in the middle layer of the indirect test procedure. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80, depending on the method used. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - 说明
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Target Species of Antibody: Monkey
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, pH 7.2. No preservative added.
- 储存液
- Without preservative
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- 抗原
- IgA
- Abstract
- IgA 产品
- 物质类
- Antibody
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