The Alkaline Phosphatase anti-salmonid Ig (H chain) monoclonal antibody can be used to indirectly detect a generalized or pathogen-specific increase in immunoglobulin level. This antibody recognizes the immunoglobin heavy chain found in all Salmonid species (Salmo, Oncorhynchus and Salvelinus). This antibody can be used as a detection reagent for fish infection by either detecting a generalized increase in fish immunoglobulin level or by detecting specific antibody. For specific antibody detection, known infectious agents are used as the antigen in an ELISA assay, followed by samples of infected fish serum and then the anti-salmonid Ig monoclonal antibody. This antibody can be used in ELISA and Western blots.
纯化方法
Immunoaffinity chromatography
免疫原
affinity purified Rainbow trout and Atlantic salmon heavy and light chain immunoglobulin . Fusion Partner: X63Ag8.653 myloma cells
This antibody can be used as a detection reagent for fish infection by either detecting a generalized increase in fish immunoglobulin level or by detecting specific antibody. For specific antibody detection, known infectious agents are used as the antigen in an ELISA assay, followed by samples of infected fish serum and then the anti-salmonid Ig monoclonal antibody. This antibody can be used in ELISA and Western blots.
限制
仅限研究用
注意事项
Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Do not add Sodium azide. Dilute only prior to immediate use Each reagent is stable for the period shown on the bottle label if stored as directed.