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小鼠 anti-人 IgM (Fc5mu Region) Antibody

ELISA, WB Monoclonal 22G1-H8 IgG2b kappa unconjugated
产品编号 ABIN1607857
发货至: 中国
  • 抗原 See all IgM products
    IgM
    抗原表位
    • 141
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    Fc5mu Region
    适用
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    小鼠
    克隆类型
    • 496
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    单克隆
    应用范围
    • 334
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    ELISA, Western Blotting (WB)
    特异性
    IgM Fc5µ
    交叉反应
    纯化方法
    Anti-Human IgM Fc5μ Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgM, Human Serum and Human Fc5μ.  No reaction was observed against Human IgG, Human IgA, Human Fc, Human Fab or Human Fab2.
    免疫原

    Immunogen: Anti-Human IgM Fc5mu was produced by repeated immunization with Human IgM Fc5mu fragment in mice.

    Immunogen Type: Native Protein

    克隆位点
    22G1-H8
    亚型
    IgG2b kappa
  • 应用备注

    Application Note: Human IgM Fc5μ antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

    ELISA Dilution: 1:1000-1:10000

    Western Blot Dilution: 1:1000-1:2000

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid cycles of freezing and thawing.
    Centrifuge product if not completely clear after standing at room temperature.
    储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 4 °C as an undiluted liquid.
    有效期
    12 months
  • 抗原
    IgM
    Abstract
    IgM 产品
    物质类
    Antibody
    背景

    Synonyms: mouse anti-Human IgM Fc5μ Antibody, Human IgM Fc5u Antibody, Ms-a-Human IgM Fc5μ Antibody, Human IgM Fc5μ Antibody in Mouse, Human M Fc5μ Secondary Antibody

    Background: Anti-Human IgM Fc5μ antibody generated in mice specifically detects Fc5μ portion of the human IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Due to this large size, IgM is typically isolated to the serum. Anti-Human IgM Fc5μ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

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