After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY fraction prepared. The antibodies were immunoaffinity purified from a solid phase immunoabsorbent and conjugated to Biotin.
纯化方法
egg yolk-derived purified IgY, affinity purified
免疫原
Chickens were immunized with highly purified bovine IgG isolated from normal pooled bovine serum.