山羊 anti-小鼠 IgG2b (Heavy Chain) Antibody (Texas Red (TR)) - Preadsorbed
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- 抗原 See all IgG2b products
- IgG2b
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抗原表位
- Heavy Chain
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Texas Red (TR)
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应用范围
- Immunohistochemistry (IHC), Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- 特异性
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Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2b.
Specificity was confirmed by ELISA at less than 0.5 % of target signal. - 交叉反应
- 小鼠
- 产品特性
- This product is suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Mouse IgG2b heavy chain
Immunogen Type: Native Protein
- 亚型
- IgG2b
- Labeling Ratio
- 2.1
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Goat anti-Mouse IgG2b (Heavy Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Mouse IgG2b (Heavy Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
Goat anti-Mouse IgG2b (Heavy Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Rat anti-Mouse IgG2b antibody (PE)ELISA, FACS, FLISA Monoclonal SB74g IgG2b PE
Goat anti-Mouse IgG2b antibody (Alkaline Phosphatase (AP))ELISA, FLISA Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Mouse IgG2b antibodyELISA, FLISA Polyclonal IgG unconjugated
Goat anti-Mouse IgG2b (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Rabbit anti-Mouse IgG2b (Heavy Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated
Rat anti-Mouse IgG2b antibodyELISA, FACS, FLISA Monoclonal SB74g IgG2b unconjugated
Goat anti-Mouse IgG2b (Heavy Chain) antibody (TRITC) - PreadsorbedELISA, IHC, WB, FLISA, FM Polyclonal IgG TRITC
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Mouse IgG2b Texas Red™ Conjugated antibodies are suitable for ELISA, Immunohistochemistry, western blotting, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting as well as other anti IgG2a antibody based assays. Mouse IgG2b secondary antibody is available in a variety of formats. This product is suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Western Blot Dilution: 1:4,000 - 1:20,000
IF Microscopy Dilution: 1:1,000 - 1:5,000
- 说明
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Texas Red™ is a registered trademark of Molecular Probes Inc.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Product is photosensitive and should be protected from light.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at -20 °C prior to opening. This product is stable for several weeks at 0 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -20 °C or below.
- 有效期
- 12 months
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- 抗原
- IgG2b
- Abstract
- IgG2b 产品
- 物质类
- Antibody
- 背景
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Synonyms: goat anti-MOUSE IgG2b Antibody Texas Red™ Conjugation, goat anti-Mouse IgG2b (gamma 2b) Texas Red™ Conjugated Antibody
Background: Anti-Mouse IgG2b Texas Red Antibody generated in goat detects reactivity to Mouse IgG2b (Gamma 2b chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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