山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody (DyLight 550)
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- 抗原 See all IgG products
- IgG
- 片段
- F(ab')2 fragment
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抗原表位
- Heavy & Light Chain
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适用
- 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- DyLight 550
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应用范围
- Immunoassay (IA)
- 特异性
- Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG and light chains on all rat immunoglobulins.
- 交叉反应
- 人, 小鼠
- 产品特性
- Goat anti-Rat IgG (H&L), F(ab) '2 fragment - DyLight® 550 Conjugated, is a secondary antibody conjugated to DyLight® 550, which binds to all rat IgG (H&L), F(ab) fragment in immunological assays. DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rat IgG (H&L).
- 纯化方法
- Affinity purification
- 免疫原
- Purified Rat IgG, whole molecule
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Rabbit anti-Rat IgG (Heavy & Light Chain) antibody (HRP)
IA Polyclonal IgG HRP
Goat anti-Rat IgG (Heavy & Light Chain) antibody (HRP)IA Polyclonal IgG HRP
Rabbit anti-Rat IgG (Heavy & Light Chain) antibody (HRP)IA Polyclonal IgG HRP
Goat anti-Rat IgG (Fc Region) antibody (HRP)IA Polyclonal IgG HRP
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- 应用备注
- 1:20 - 1:2,000 for most applications
- 说明
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Based on immunoelectrophoresis, this antibody reacts with: heavy (gamma) chains on rat IgG light chains on all rat immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins IgG from human or mouse.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution please add 0.55 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
- 注意事项
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Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. - 储存条件
- 4 °C/-20 °C
- 储存方法
- Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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