小鼠 anti-人 IgA + IgD + IgE + IgG + IgM Antibody
IF, ELISA
Polyclonal
unconjugated
-
- 抗原
- IgA + IgD + IgE + IgG + IgM
- 适用
- 人
- 宿主
- 小鼠
- 克隆类型
- 多克隆
- 应用范围
- Immunofluorescence (IF), ELISA
- 产品特性
-
The reactivity of the antibody is restricted to a isotype specific determinant as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic immunoglobulins.
Monoclonal antibody concentration in the solution is 1.0 mg/mL.
Physical form: Delipidated, heat inactivated, lyophilized stable ascites. - 纯化方法
- Ascites
- 免疫原
- Highly purified monoclonal IgG subclasses isolated from pooled human serum.
-
- 应用备注
- The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly form 1:100 to 1:500, in ELISA from 1:1,000 upwards, in Western blotting 1:2,000 upwards. These data should be interpreted as general recommendations only.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
- 缓冲液
- No foreign proteins added.
- 注意事项
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not refrozen, and preferably used the same day.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
-
- 抗原
- IgA + IgD + IgE + IgG + IgM
- 背景
- To identify the presence of immunoglobulin classes in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, subclasses and immunoblotting.
-
You are here:
