山羊 anti-小鼠 IgG, IgM (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed
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- 抗原 See all IgG, IgM products
- IgG, IgM
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抗原表位
- Heavy & Light Chain
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Alkaline Phosphatase (AP)
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, Mouse IgG and Mouse IgM.
- 交叉反应
- 小鼠
- 纯化方法
- Preadsorption: Solid phase absorption
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Mouse IgG and IgM whole molecules
- 亚型
- IgG
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- 应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Mouse IgG +IgM Alk Phos conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
ELISA Dilution: 1:2,000 - 1:15,000
Western Blot Dilution: 1:500 - 1:2,500
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.1 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.
Centrifuge product if not completely clear after standing at room temperature. - 储存条件
- 4 °C
- 储存方法
- Store vial at 4 °C prior to opening.
- 有效期
- 12 months
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- 抗原
- IgG, IgM
- 别名
- IgG + IgM (IgG, IgM 产品)
- 物质类
- Antibody
- 背景
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Synonyms: Goat Anti-Mouse IgG + IgM Antibody alkaline phosphatase Conjugated Pre-Adsorbed, Goat Anti-Mouse IgG/IgM Antibody alk phos Conjugated
Background: Anti-Mouse IgG + IgM Alkaline Phosphatase Antibody generated in Goat detects reactivity to Mouse IgG and IgM. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG + IgM Alkaline Phosphate Antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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