驴 anti-豚鼠 IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed
-
- 抗原 See all IgG products
- IgG
-
抗原表位
- Heavy & Light Chain
-
适用
- 豚鼠
-
宿主
- 驴
-
克隆类型
- 多克隆
-
标记
- Alkaline Phosphatase (AP)
-
应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Guinea Pig IgG and Guinea Pig Serum.
- 交叉反应
- 豚鼠
- 纯化方法
- Preadsorption: immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads
- 过滤
- Sterile filtered
- 免疫原
-
Immunogen: Guinea Pig IgG whole molecule
- 亚型
- IgG
-
Goat anti-Guinea Pig IgG (Heavy & Light Chain) antibody (HRP) - Preadsorbed
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Donkey anti-Guinea Pig IgG (Heavy & Light Chain) antibody - PreadsorbedELISA, WB Polyclonal IgG unconjugated
Goat anti-Guinea Pig IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, WB Polyclonal IgG HRP
Rabbit anti-Guinea Pig IgG (Heavy & Light Chain) antibody (HRP)ELISA Polyclonal IgG HRP
Donkey anti-Guinea Pig IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin
Goat anti-Guinea Pig IgG (Fc Region) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Guinea Pig IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
-
- 应用备注
-
Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Guinea Pig IgG Alk Phos conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
ELISA Dilution: 1:2,000 - 1:10,000
Western Blot Dilution: 1:500 - 1:2,500
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
-
Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.1 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
-
Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.
Centrifuge product if not completely clear after standing at room temperature. - 储存条件
- 4 °C
- 储存方法
- Store vial at 4 °C prior to restoration. This product is stable for several weeks at 4 °C as an undiluted liquid.
- 有效期
- 12 months
-
- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
-
Synonyms: Donkey Anti-Guinea Pig IgG alkaline phosphatase Conjugation, Donkey Anti-Guinea Pig IgG alk phos Conjugated
Background: Anti-Guinea Pig IgG Alkaline Phosphatase Antibody generated in donkey detects guinea pig IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
-
