(1 reference)-
- 抗原 See all IgM products
- IgM
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抗原表位
- Heavy Chain
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- IgM µ chain
- 产品特性
- Concentration Definition: by Refractometry
- 纯化方法
- This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Rat IgM and Rat Serum. No reaction was observed against Rat IgG F(ab')2 or Rat IgG F(c).
- 免疫原
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Immunogen: Rat IgM mu heavy chain
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Goat anti-Rat IgM (Heavy Chain) antibody
ELISA, IHC, FACS, WB Polyclonal IgG unconjugated
Goat anti-Rat IgM (Heavy Chain) antibody (Texas Red (TR))ELISA, IHC, FACS Polyclonal IgG Texas Red (TR)
Goat anti-Rat IgM (Heavy Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
Goat anti-Rat IgM (Heavy Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Rat IgM (Heavy Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rat IgM (Heavy Chain) antibody (Biotin)ELISA, IHC, FACS, WB Polyclonal IgG Biotin
Goat anti-Rat IgM (Heavy Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated
Rabbit anti-Rat IgM (Heavy Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated
Goat anti-Rat IgM (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Mouse anti-Rat IgM antibodyELISA, FACS Monoclonal M2A1 IgG1 unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Rat IgM antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 90 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 有效期
- 12 months
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: "Role of IgM and angiotensin II Type I receptor autoantibodies in local complement activation in placental ischemia-induced hypertension in the rat." in: Molecular immunology, Vol. 78, pp. 38-47, (2017) (PubMed).
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: "Role of IgM and angiotensin II Type I receptor autoantibodies in local complement activation in placental ischemia-induced hypertension in the rat." in: Molecular immunology, Vol. 78, pp. 38-47, (2017) (PubMed).
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
- 背景
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Synonyms: rabbit anti-Rat IgM (mu) Antibody, rabbit anti Rat IgM mu
Background: Anti-Rat IgM antibody generated in rabbit specifically detects rat IgM mu heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Rat IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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