山羊 anti-小鼠 IgM (Heavy & Light Chain) Antibody
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- 抗原 See all IgM products
- IgM
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抗原表位
- Heavy & Light Chain
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
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IgM (H&L)
Mouse IgM antibody recognizes the mu chain of the Mouse IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Mouse IgM and Mouse Serum. ELISA was used to confirm specificity at less than 1 % cross reactivity against other mouse heavy or light chain isotypes. - 产品特性
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Anti-Mouse IgM antibody is ideal with investigators involved in serum component protein research.
Concentration Definition: by UV absorbance at 280 nm - 纯化方法
- Anti-MOUSE IgM (H&L) (GOAT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum. Some light chain cross reactivity may occur against Mouse IgG.
- 免疫原
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Immunogen: Mouse IgM whole molecule
- 亚型
- IgG
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Goat anti-Mouse IgM (Heavy Chain) antibody (TRITC)
ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Mouse IgM (Heavy Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
Goat anti-Mouse IgM (Heavy Chain) antibody (Biotin)ELISA, IHC, FACS, WB Polyclonal IgG Biotin
Goat anti-Mouse IgM (Heavy Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Mouse IgM antibody (APC) - PreadsorbedELISA, FACS Polyclonal IgG APC
Goat anti-Mouse IgM antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE
Goat anti-Mouse IgM (Heavy Chain) antibodyELISA, IHC, FACS, WB Polyclonal IgG unconjugated
Goat anti-Mouse IgM (Heavy & Light Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Mouse IgM (mu chain) (GOAT) Antibody has been assayed against Mouse IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin. A working dilution of 1:15,000 to 1:70,000 of the concentration is suggested for this product. Mouse IgM antibody is suitable for immunoassays including western blot and ELISA.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 5.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 10.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 有效期
- 12 months
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
- 背景
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Synonyms: Mouse antibody, goat anti mouse, IgM, immunoglobulin fraction, goat anti-mouse IgM antibody
Background: Anti-Mouse IgM antibody specifically detects mouse IgM. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Mouse IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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