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山羊 anti-人 IgM (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
产品编号 ABIN102625
发货至: 中国
  • 抗原 See all IgM products
    IgM
    抗原表位
    • 141
    • 118
    • 87
    • 40
    • 17
    • 8
    • 7
    • 1
    • 1
    Heavy & Light Chain
    适用
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    宿主
    • 320
    • 188
    • 75
    • 15
    • 14
    • 5
    • 4
    • 1
    • 1
    山羊
    克隆类型
    • 495
    • 80
    多克隆
    标记
    • 121
    • 77
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    • 55
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    • 32
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    • 2
    • 2
    • 1
    • 1
    • 1
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    • 1
    • 1
    Alkaline Phosphatase (AP)
    应用范围
    • 334
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    • 139
    • 64
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    IgM µ chain
    交叉反应
    产品特性
    Anti-Human IgM mu heavy chain antibody generated in goat detects specifically Human IgM mu heavy chain. This secondary antibody anti-Human is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: Solid phase absorption
    过滤
    Sterile filtered
    免疫原

    Immunogen: Human IgM whole molecule

    Immunogen Type: Native Protein

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:200 - 1:1,000

    Application Note: Anti-Human IgM (mu heavy chain) is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.

    ELISA Dilution: 1:2,000 - 1:10,000

    Western Blot Dilution: 1:500 - 1:2,500

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Do not add Sodium azide.
    Dilute only prior to immediate use
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    储存条件
    4 °C
    有效期
    12 months
  • 抗原
    IgM
    Abstract
    IgM 产品
    物质类
    Antibody
    背景

    Synonyms: goat anti-Human IgM mu heavy chain Antibody, Gt-a-Human IgM mu heavy chain, Human IgM mu heavy chain Antibody in Goat, Human IgM mu heavy chain Secondary Antibody

    Background: Anti-Human IgM (mu heavy chain) Alkaline Phosphatase generated in goat detects specifically Human IgM mu heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Anti-Human IgM mu chain antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

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