Purified recombinant Human IFN gamma protein Expression System: E.coli Bioactivity: The ED50 as determined by a cytopathic affect inhibition assay with Mouse L929 cells challenged with EMC virus was 1 x 10^7 units/mg.
IFNG
宿主: 大鼠
宿主: HEK-293 Cells
Recombinant
> 92 % as determined by SDS-PAGE
Active
应用备注
Each Investigator should determine their own optimal working dilution for specific applications.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute in 10 mM Na0 O4 pH7.2 to a concentration of 0.1-1.0 mg/mL. When dissolved, dilute further into aqueous buffered solutions.
缓冲液
Lyophilized from 10 mM Na3PO4, pH7.2.
注意事项
Avoid repeated freeze/thaw cycles.
储存条件
RT/-20 °C
POGO, BRAWERMAN, CHARGAFF: "New ribonucleic acid species associated wihin the formation of the photosynthetic apparatus in Euglena gracilis." in: Biochemistry, Vol. 1, pp. 128-31 (PubMed).
IFN-gamma is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-gamma receptors are present in most immune cells, which respond to IFN-gamma signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-gamma signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. Additionally, IFN-gamma stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-gamma is species-specific and is biologically active only in human and primate cells. Alternative Names: IFN g protein, Immune Interferon protein, T cell interferon protein, IFN-g protein, MAF protein, type II interferon protein, Interferon gamma protein