AKR1B10 Protein (AA 1-316)
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- 抗原 See all AKR1B10 蛋白
- AKR1B10 (Aldo-Keto Reductase Family 1, Member B10 (Aldose Reductase) (AKR1B10))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-316
- 宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVR KAFEKTLKDL KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD EGLVKALGVS NFSHFQIEKL LNKPGLKYKP VTNQVECHPY LTQEKLIQYC HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK HKKTAAQVLI RFHIQRNVIV IPKSVTPARI VENIQVFDFK LSDEEMATIL SFNRNWRACN VLQSSHLEDY PFDAEY
- 产品特性
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Purified recombinant Human AKR1 B10 protein
Expression System: E.coli
Bioactivity: Specific activity: approximately 0.32 - 0.4 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 uM NADPH per minute at 25 °C. Specific activity was expressed as units/mg protein.
Molecular weight on SDS-PAGE will appear higher. - 纯度
- > 95 % pure
- Top Product
- Discover our top product AKR1B10 蛋白
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Aldo-Keto Reductase Family 1, Member B10 (Aldose Reductase) (AKR1B10) (AA 1-316) protein (GST tag)
AKR1B10 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Aldo-Keto Reductase Family 1, Member B10 (Aldose Reductase) (AKR1B10) (AA 1-316) proteinAKR1B10 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Aldo-Keto Reductase Family 1, Member B10 (Aldose Reductase) (AKR1B10) (AA 76-143) protein (GST tag)AKR1B10 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Aldo-Keto Reductase Family 1, Member B10 (Aldose Reductase) (AKR1B10) (AA 2-316) protein (His tag)Crystallography grade AKR1B10 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Aldo-Keto Reductase Family 1, Member B10 (Aldose Reductase) (AKR1B10) protein (Myc-DYKDDDDK Tag)AKR1B10 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- AKR1B10 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- 实验流程
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- Prepare a 1 mL reaction mix into a suitable container: The final concentrations are 0.1 M sodium phosphate (pH 7.0), 11 mM DL-glyceraldehyde, 0.1 mM NADPH.
2. Add 50 µL of recombinant AKR1B10 solution with various concentrations (2.5 µg, 5 µg, 15 µg) in 750 µL reaction buffer .
3. Mix by inversion and Incubate at 25 °C for
2.5 minutes.
4. Add 200 µL of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion.
5. Record the increase at A340 nm for 3 minutes.
- Prepare a 1 mL reaction mix into a suitable container: The final concentrations are 0.1 M sodium phosphate (pH 7.0), 11 mM DL-glyceraldehyde, 0.1 mM NADPH.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 10 % glycerol.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- RT/-20 °C
- 储存方法
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- 抗原
- AKR1B10 (Aldo-Keto Reductase Family 1, Member B10 (Aldose Reductase) (AKR1B10))
- 别名
- AKR1B10 (AKR1B10 产品)
- 别名
- AKR1B11 Protein, AKR1B12 Protein, ALDRLn Protein, ARL-1 Protein, ARL1 Protein, HIS Protein, HSI Protein, 2310005E10Rik Protein, Akr1b16 Protein, AKR Protein, AKR1B10 Protein, Akr1b10 Protein, aldo-keto reductase family 1 member B10 Protein, aldo-keto reductase family 1, member B10 (aldose reductase) Protein, aldo-keto reductase family 1 member B10-like 2 Protein, AKR1B10 Protein, Akr1b10 Protein, AKR1B10L2 Protein, LOC100585902 Protein, LOC100723118 Protein, LOC101107697 Protein
- 背景
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AKR1B10, also known as Aldo-keto reductase family 1, member B10, AKR1B10 is a monomeric protein that efficiently catalyzes the reduction of aromatic and aliphatic aldehydes and ketones. AKR1B10 is ubiquitously expressed in many human tissues but is highly expressed in small intestine, colon and adrenal gland. This protein is pathogenically involved in diabetic complications and has been reported that AKR1B10 is overexpressed in human tumors, such as liver, breast, and lung cancer, and may play a critical role in the development and progression of cancer. Recombinant human AKR1B10 protein was expressed in E. coli and purified by using conventional chromatography.
Alternative Names: AKRB0 1, AKR1B12 protein, member B10 protein, AKRB0-1 protein, AKR1B10 protein, HIS protein, SI reductase protein, HIS protein, Aldose reductase related protein protein, AKR1B10, AKRB0-1, ARL1 protein, Aldo-keto reductase family 1 protein, Small intestine reductase., member B10 Aldose reductase like protein, ARL-1 protein, ALDRLn protein, AKRB0 1 protein, hARP protein, ARL 1 protein, AKR1B11 protein, Aldo-keto reductase family 1 protein - 分子量
- 36 kDa (316 AA)
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