AKR1B1 Protein (AA 1-316)
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- 抗原 See all AKR1B1 蛋白
- AKR1B1 (Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-316
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF
- 产品特性
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Purified recombinant Human Aldose reductase protein
Expression System: E.coli
Bioactivity: Specific activity: approximately 0.5-0.6 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 uM NADPH/min at 25 °C.
Molecular weight on SDS-PAGE will appear higher. - 纯度
- > 95 % pure
- 内毒素水平
- < 1.0 EU per µg of protein (determined by LAL method)
- Top Product
- Discover our top product AKR1B1 蛋白
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Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1) (AA 1-316) protein (GST tag)
AKR1B1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1) (AA 1-316) proteinAKR1B1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1) (AA 1-316) protein (His tag)AKR1B1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1) (AA 217-316) protein (GST tag)AKR1B1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1) (AA 1-316) (Active) protein (His tag)AKR1B1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS Active
Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1) protein (Myc-DYKDDDDK Tag)AKR1B1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Aldose reductase protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- 实验流程
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- Prepare a 1 mL reaction mix into a suitable container : The final concentrations are 0.1 M sodium phosphate (pH 7.0), 11 mM DL-glyceraldehyde, 0.1 mM NADPH.
2. Add 50 µL of recombinant AKR1B1 solution with various concentrations (2.5 µg, 5 µg, 15 µg) in 750 µL reaction buffer.
3. Mix by inversion and Incubate at 25 °C for
2.5 minutes.
4. Add 200 µL of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion.
5. Record the increase at A340 nm for 3 minutes.
- Prepare a 1 mL reaction mix into a suitable container : The final concentrations are 0.1 M sodium phosphate (pH 7.0), 11 mM DL-glyceraldehyde, 0.1 mM NADPH.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT, and 10 % glycerol.
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- RT/-20 °C
- 储存方法
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- 抗原
- AKR1B1 (Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1))
- 别名
- Aldose reductase (AKR1B1 产品)
- 别名
- ADR Protein, ALDR1 Protein, ALR2 Protein, AR Protein, ALDRED Protein, ALR-P-I Protein, Akr1b3 Protein, Akr1b4 Protein, Aldr1 Protein, Alr Protein, RATALDRED Protein, akr1b7 Protein, zgc:86611 Protein, aldo-keto reductase family 1 member B Protein, aldo-keto reductase family 1, member B1 (aldose reductase) Protein, aldo-keto reductase family 1, member B1 (aldose reductase) S homeolog Protein, AKR1B1 Protein, Akr1b1 Protein, akr1b1.S Protein, akr1b1 Protein
- 背景
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Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Recombinant Aldose reductase (AKR1B) protein was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: ALR2 protein, member B1 AKR1B 1 protein, ALDR1 protein, aldehyde reductase 1 protein, aldo-keto reductase family 1 protein, AKR1B1 protein, Aldo-keto reductase family1 member B1 protein, AR protein, aldr-1 protein, Lii5-2 CTCL tumor protein protein, ADR protein, Aldose reductase protein, member B1 aldr 1 protein, Aldo-keto reductase family1 protein, low Km aldose reductase., Aldehyde reductase protein - 分子量
- 35.8 kDa (316 AA)
- 途径
- Metabolism of Steroid Hormones and Vitamin D, C21-Steroid Hormone Metabolic Process, Monocarboxylic Acid Catabolic Process
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